primary antibodies targeting caveolin 1 Search Results


primary antibodies caveolin-1  (Becton Dickinson)
90
Becton Dickinson primary antibodies caveolin-1
(A) Immunogold staining of dynamin2 on plasma membrane lawns prepared from 3T3-L1 cells. (gold particles 15nm). Caveolae in white boxes are shown at higher magnification in right panels; clathrin coated pit marked with asterisk. (B) Representative image from TIRF movie of <t>Cav1-mCh</t> cell. Scale bar, 10 μm. Color-coded trajectories illustrate the time and displacement length of tracked caveolae structures during 5 minutes. 1 in red represents caveolae with long duration time, short displacement length and low speed. 2 in cyan represents caveolae with long duration time, short displacement length and low speed. 3 in blue represents caveolae with short duration time, short displacement length and high speed. 4 in purple represents caveolae with short duration time, intermediate displacement length and high speed. (C-H) Cav1-mCh cells were transfected with siRNA for 72 hours before experiments were performed. (C) Representative immunoblots of cells treated with siRNAs as indicated. n=3 (D) Number of caveolae counted in the basal plasma membrane in cells imaged on TIRF. Mean ± SEM from at least 17 cells per condition. (E) Color-coded trajectories of caveolae in Cav1-mCh cells treated with siRNA as indicated. Cells were imaged by TIRF over 5 minutes. (F) Quantification of Cav1-mCh track duration time (G) speed and (H) displacement length. Numbers were related to control (Ctrl) treated cells. Analyses were performed using Imaris software and track mean ± SEM from at least 8 cells per condition are shown. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01, *** p ≤0.001.
Primary Antibodies Caveolin 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+targeting+caveolin+1/primary+antibodies+against+caveolin+1/bio_rxiv__2022__05__25__493372-282-7-8
Average 90 stars, based on 1 article reviews
primary antibodies caveolin-1 - by Bioz Stars, 2026-10
90/100 stars
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primary antibodies caveolin-1  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company primary antibodies caveolin-1
(A) Immunogold staining of dynamin2 on plasma membrane lawns prepared from 3T3-L1 cells. (gold particles 15nm). Caveolae in white boxes are shown at higher magnification in right panels; clathrin coated pit marked with asterisk. (B) Representative image from TIRF movie of <t>Cav1-mCh</t> cell. Scale bar, 10 μm. Color-coded trajectories illustrate the time and displacement length of tracked caveolae structures during 5 minutes. 1 in red represents caveolae with long duration time, short displacement length and low speed. 2 in cyan represents caveolae with long duration time, short displacement length and low speed. 3 in blue represents caveolae with short duration time, short displacement length and high speed. 4 in purple represents caveolae with short duration time, intermediate displacement length and high speed. (C-H) Cav1-mCh cells were transfected with siRNA for 72 hours before experiments were performed. (C) Representative immunoblots of cells treated with siRNAs as indicated. n=3 (D) Number of caveolae counted in the basal plasma membrane in cells imaged on TIRF. Mean ± SEM from at least 17 cells per condition. (E) Color-coded trajectories of caveolae in Cav1-mCh cells treated with siRNA as indicated. Cells were imaged by TIRF over 5 minutes. (F) Quantification of Cav1-mCh track duration time (G) speed and (H) displacement length. Numbers were related to control (Ctrl) treated cells. Analyses were performed using Imaris software and track mean ± SEM from at least 8 cells per condition are shown. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01, *** p ≤0.001.
Primary Antibodies Caveolin 1, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+targeting+caveolin+1/primary+antibodies+caveolin+1/pm26768616-58-8-16
Average 90 stars, based on 1 article reviews
primary antibodies caveolin-1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


(A) Immunogold staining of dynamin2 on plasma membrane lawns prepared from 3T3-L1 cells. (gold particles 15nm). Caveolae in white boxes are shown at higher magnification in right panels; clathrin coated pit marked with asterisk. (B) Representative image from TIRF movie of Cav1-mCh cell. Scale bar, 10 μm. Color-coded trajectories illustrate the time and displacement length of tracked caveolae structures during 5 minutes. 1 in red represents caveolae with long duration time, short displacement length and low speed. 2 in cyan represents caveolae with long duration time, short displacement length and low speed. 3 in blue represents caveolae with short duration time, short displacement length and high speed. 4 in purple represents caveolae with short duration time, intermediate displacement length and high speed. (C-H) Cav1-mCh cells were transfected with siRNA for 72 hours before experiments were performed. (C) Representative immunoblots of cells treated with siRNAs as indicated. n=3 (D) Number of caveolae counted in the basal plasma membrane in cells imaged on TIRF. Mean ± SEM from at least 17 cells per condition. (E) Color-coded trajectories of caveolae in Cav1-mCh cells treated with siRNA as indicated. Cells were imaged by TIRF over 5 minutes. (F) Quantification of Cav1-mCh track duration time (G) speed and (H) displacement length. Numbers were related to control (Ctrl) treated cells. Analyses were performed using Imaris software and track mean ± SEM from at least 8 cells per condition are shown. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01, *** p ≤0.001.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Immunogold staining of dynamin2 on plasma membrane lawns prepared from 3T3-L1 cells. (gold particles 15nm). Caveolae in white boxes are shown at higher magnification in right panels; clathrin coated pit marked with asterisk. (B) Representative image from TIRF movie of Cav1-mCh cell. Scale bar, 10 μm. Color-coded trajectories illustrate the time and displacement length of tracked caveolae structures during 5 minutes. 1 in red represents caveolae with long duration time, short displacement length and low speed. 2 in cyan represents caveolae with long duration time, short displacement length and low speed. 3 in blue represents caveolae with short duration time, short displacement length and high speed. 4 in purple represents caveolae with short duration time, intermediate displacement length and high speed. (C-H) Cav1-mCh cells were transfected with siRNA for 72 hours before experiments were performed. (C) Representative immunoblots of cells treated with siRNAs as indicated. n=3 (D) Number of caveolae counted in the basal plasma membrane in cells imaged on TIRF. Mean ± SEM from at least 17 cells per condition. (E) Color-coded trajectories of caveolae in Cav1-mCh cells treated with siRNA as indicated. Cells were imaged by TIRF over 5 minutes. (F) Quantification of Cav1-mCh track duration time (G) speed and (H) displacement length. Numbers were related to control (Ctrl) treated cells. Analyses were performed using Imaris software and track mean ± SEM from at least 8 cells per condition are shown. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01, *** p ≤0.001.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Staining, Transfection, Western Blot, Software

Representative immunoblot of Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Doxycycline concentration used for further experiments were 1.0 ng/ml. (B) Representative image from TIRF movie of Dyn2-GFP-Cav1-mCh cell. Red arrow highlight structure only positive for Cav1-mCh, yellow arrow highlight structure positive for both Cav1-mCh and Dyn2-GFP and green arrow highlight structures only positive for Dyn2-GFP. Scale bar, 10 μm. (C) Quantification of percentage of caveolae positive for Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (D) Top, TIRF image series of stable caveolae positive for both Cav1-mCh and Dyn2-GFP. Time in sec as indicated. Bottom, kymograph showing stable association of Cav1-mCh and Dyn2-GFP to the plasma membrane during 300 sec in TIRF movie. (E) Relative fluorescence intensity of mCh and GFP fluorophore of caveolae positive for both Cav1-mCh and Dyn2-GFP over time. (F) Quantification of Cav1-mCh track duration time, displacement length and mean speed in Cav1-mCh cells (grey) and Dyn2-GFP-Cav1-mCh cells (black). Analysis were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. (G) Caveolae track duration times and displacement lengths divided in pools of Dyn2-GFP negative (red) or positive (orange). Dyn2-GFP-Cav1-mCh cells were imaged on TIRF over 5 minutes. Cav1-mCh spots were followed throughout the time series and scored whether they were positive or negative for Dyn2-GFP. Scatter dot plots show all Cav1-mCh tracks from three different cells. Analysis were performed using Imaris software and data are shown as mean ± SEM. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: Representative immunoblot of Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Doxycycline concentration used for further experiments were 1.0 ng/ml. (B) Representative image from TIRF movie of Dyn2-GFP-Cav1-mCh cell. Red arrow highlight structure only positive for Cav1-mCh, yellow arrow highlight structure positive for both Cav1-mCh and Dyn2-GFP and green arrow highlight structures only positive for Dyn2-GFP. Scale bar, 10 μm. (C) Quantification of percentage of caveolae positive for Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (D) Top, TIRF image series of stable caveolae positive for both Cav1-mCh and Dyn2-GFP. Time in sec as indicated. Bottom, kymograph showing stable association of Cav1-mCh and Dyn2-GFP to the plasma membrane during 300 sec in TIRF movie. (E) Relative fluorescence intensity of mCh and GFP fluorophore of caveolae positive for both Cav1-mCh and Dyn2-GFP over time. (F) Quantification of Cav1-mCh track duration time, displacement length and mean speed in Cav1-mCh cells (grey) and Dyn2-GFP-Cav1-mCh cells (black). Analysis were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. (G) Caveolae track duration times and displacement lengths divided in pools of Dyn2-GFP negative (red) or positive (orange). Dyn2-GFP-Cav1-mCh cells were imaged on TIRF over 5 minutes. Cav1-mCh spots were followed throughout the time series and scored whether they were positive or negative for Dyn2-GFP. Scatter dot plots show all Cav1-mCh tracks from three different cells. Analysis were performed using Imaris software and data are shown as mean ± SEM. Significance was assessed using t test, * p ≤0.05, ** p ≤0.01.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Western Blot, Concentration Assay, Fluorescence, Software

(A) Immunogold labelling of EHD2 (large gold) and Dyn2 (small gold) on plasma membrane lawns prepared from 3T3-L1 cells. Red indicates caveolae and green clathrin coated pits. (B) Representative image from TIRF movie of Dyn2-GFP-Cav1-mCh cells transiently expressing EHD2-BFP. White arrows highlight structures where all three tagged proteins are colocalized and green arrows highlights structures only positive for Dyn2-GFP. Scale bar, 10 μm. (C) Recovery curves of Dyn2-GFP intensities after photobleaching of caveolae in Dyn2-GFP-Cav1-mCh cells treated with Ctrl, EHD2 or pacsin2 siRNA as indicated. (D) Image panel showing GFP recovery after photobleaching in Dyn2GFP-Cav1-mCh cells, GFP-EHD2-Cav1-mCh cells or Pac2-GFP-Cav1-mCh cells. Graph shows percentage GFP-intensity recovery curves after photobleaching of mCh and GFP colocalizing structures at the PM. (E) Quantification of Cav1-mCh track duration time and displacement length in GFP-EHD2-Cav1-mCh cells (green). Numbers were related to Cav1-mCh cells (white). Track mean ± SEM from at least 7 cells per condition are shown. (F) Quantification of Cav1-mCh track duration time and displacement length in Dyn2-GFP-Cav1-mCh cells (black), transiently expressing EHD2-BFP (dark green) or depleted from EHD2 (purple). Numbers were related to Dyn2-GFP-Cav1-mCh cells (black). Track mean ± SEM from at least 7 cells per condition are shown. Significance was assessed using t test, ** p ≤0.01, *** p ≤0.001.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Immunogold labelling of EHD2 (large gold) and Dyn2 (small gold) on plasma membrane lawns prepared from 3T3-L1 cells. Red indicates caveolae and green clathrin coated pits. (B) Representative image from TIRF movie of Dyn2-GFP-Cav1-mCh cells transiently expressing EHD2-BFP. White arrows highlight structures where all three tagged proteins are colocalized and green arrows highlights structures only positive for Dyn2-GFP. Scale bar, 10 μm. (C) Recovery curves of Dyn2-GFP intensities after photobleaching of caveolae in Dyn2-GFP-Cav1-mCh cells treated with Ctrl, EHD2 or pacsin2 siRNA as indicated. (D) Image panel showing GFP recovery after photobleaching in Dyn2GFP-Cav1-mCh cells, GFP-EHD2-Cav1-mCh cells or Pac2-GFP-Cav1-mCh cells. Graph shows percentage GFP-intensity recovery curves after photobleaching of mCh and GFP colocalizing structures at the PM. (E) Quantification of Cav1-mCh track duration time and displacement length in GFP-EHD2-Cav1-mCh cells (green). Numbers were related to Cav1-mCh cells (white). Track mean ± SEM from at least 7 cells per condition are shown. (F) Quantification of Cav1-mCh track duration time and displacement length in Dyn2-GFP-Cav1-mCh cells (black), transiently expressing EHD2-BFP (dark green) or depleted from EHD2 (purple). Numbers were related to Dyn2-GFP-Cav1-mCh cells (black). Track mean ± SEM from at least 7 cells per condition are shown. Significance was assessed using t test, ** p ≤0.01, *** p ≤0.001.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Expressing

(A) Confocal image of a Dyn2-GFP-Cav1-mCh cell transiently expressing Δ NΔEH EHD2-BFP. Yellow arrow highlights Dyn2-GFP localization to Cav1-mCh at the tip of the membrane tube. Scale bar, 10 μm. (B) Apparent FRET efficiency between GFP and mCh fluorophores in GFP-EHD2-Cav1-mCh cells, Dyn2-GFP-Cav1-mCh cells or Cav1-mCh transiently expressing Cavin1-GFP. ROIs of structures positive for only GFP (green) or GFP and Cav1 (orange) were used to calculate the apparent FRET efficiency. Significance was assessed using t test, **** p ≤0.0001.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Confocal image of a Dyn2-GFP-Cav1-mCh cell transiently expressing Δ NΔEH EHD2-BFP. Yellow arrow highlights Dyn2-GFP localization to Cav1-mCh at the tip of the membrane tube. Scale bar, 10 μm. (B) Apparent FRET efficiency between GFP and mCh fluorophores in GFP-EHD2-Cav1-mCh cells, Dyn2-GFP-Cav1-mCh cells or Cav1-mCh transiently expressing Cavin1-GFP. ROIs of structures positive for only GFP (green) or GFP and Cav1 (orange) were used to calculate the apparent FRET efficiency. Significance was assessed using t test, **** p ≤0.0001.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Expressing

(A) Representative image from TIRF movie of K44A Dyn2-GFP-Cav1-mCh cell. Red arrow highlight structure only positive for Cav1-mCh, yellow arrow highlight structure positive for both Cav1-mCh and K44A Dyn2-GFP and green arrow highlight structures only positive for K44A Dyn2-GFP. Scale bar, 10 μm. (B) Quantification of percentage of caveolae positive for K44A Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (C) Caveolae track duration times and displacement lengths divided in pools of K44A Dyn2-GFP negative (red) or positive (orange). K44A Dyn2-GFP-Cav1-mCh cells were imaged on TIRF over 5 minutes. Cav1-mCh spots were followed and scored whether they were positive or negative for Dyn2-GFP. Graph shows all Cav1-mCh tracks from three different cells. Analysis were performed using Imaris software and data are presented as scatter dot plot, mean ± SEM. Significance was assessed using t test.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Representative image from TIRF movie of K44A Dyn2-GFP-Cav1-mCh cell. Red arrow highlight structure only positive for Cav1-mCh, yellow arrow highlight structure positive for both Cav1-mCh and K44A Dyn2-GFP and green arrow highlight structures only positive for K44A Dyn2-GFP. Scale bar, 10 μm. (B) Quantification of percentage of caveolae positive for K44A Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (C) Caveolae track duration times and displacement lengths divided in pools of K44A Dyn2-GFP negative (red) or positive (orange). K44A Dyn2-GFP-Cav1-mCh cells were imaged on TIRF over 5 minutes. Cav1-mCh spots were followed and scored whether they were positive or negative for Dyn2-GFP. Graph shows all Cav1-mCh tracks from three different cells. Analysis were performed using Imaris software and data are presented as scatter dot plot, mean ± SEM. Significance was assessed using t test.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Software

(A) Color-coded trajectories of caveolae in Dyn2-GFP-Cav1-mCh cells pretreated with DMSO (Ctrl), Dyngo 4a or Ryngo 1-23 for 30 minutes before imaged on TIRF over 5 minutes. (B) Quantification of percentage of caveolae positive for Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (C) Quantification of Cav1-mCh track duration time and displacement length in cells as indicated. Numbers were related to control (Ctrl) treated cells. Analysis were performed using Imaris software and track mean from at least 8 cells per condition are shown ± SEM. (D) Quantification of Cav1-mCh track duration time in Cav1-mCh cells treated with siRNA as indicated prior to pretreatment of DMSO (Ctrl) or Dyngo 4a as in (A). Numbers were related to DMSO-treated Ctrl siRNA-treated cells (black bar). Analysis were performed using Imaris software and track mean from at least 8 cells per condition are shown ± SEM. Significance was assessed using t test, *** p ≤0.001.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Color-coded trajectories of caveolae in Dyn2-GFP-Cav1-mCh cells pretreated with DMSO (Ctrl), Dyngo 4a or Ryngo 1-23 for 30 minutes before imaged on TIRF over 5 minutes. (B) Quantification of percentage of caveolae positive for Dyn2-GFP. Data are shown as scatter dot plot, mean ± SEM. (C) Quantification of Cav1-mCh track duration time and displacement length in cells as indicated. Numbers were related to control (Ctrl) treated cells. Analysis were performed using Imaris software and track mean from at least 8 cells per condition are shown ± SEM. (D) Quantification of Cav1-mCh track duration time in Cav1-mCh cells treated with siRNA as indicated prior to pretreatment of DMSO (Ctrl) or Dyngo 4a as in (A). Numbers were related to DMSO-treated Ctrl siRNA-treated cells (black bar). Analysis were performed using Imaris software and track mean from at least 8 cells per condition are shown ± SEM. Significance was assessed using t test, *** p ≤0.001.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Software

(A) Schematic of fusogenic liposome methodology. (B) Time series showing the incorporation of Bodipy-labelled cholesterol into the PM of a Cav1-mCh cell. Scale bar, 10 μm. (C) Quantification of track duration time in Cav1-mCh cells pretreated with Ctrl or Dyn2 siRNA as indicated. Cells were non-treated (grey bar) or treated with fusogenic liposomes containing cholesterol (black bar) for 20 minutes prior to imaging. Analyses were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. (D) Quantification of track duration time in Cav1-mCh cells, Dyn2-GFP-Cav1-mCh cells or GFP-EHD2-Cav1-mCh cells non-treated (grey bar) or treated with fusogenic liposomes containing cholesterol (black bar) for 20 minutes prior to imaging. Analysis were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. Significance was assessed using t test, ** p ≤0.01, *** p ≤0.001.

Journal: bioRxiv

Article Title: Dynamin2 stabilizes plasma membrane-connected caveolae by restraining fission

doi: 10.1101/2022.05.25.493372

Figure Lengend Snippet: (A) Schematic of fusogenic liposome methodology. (B) Time series showing the incorporation of Bodipy-labelled cholesterol into the PM of a Cav1-mCh cell. Scale bar, 10 μm. (C) Quantification of track duration time in Cav1-mCh cells pretreated with Ctrl or Dyn2 siRNA as indicated. Cells were non-treated (grey bar) or treated with fusogenic liposomes containing cholesterol (black bar) for 20 minutes prior to imaging. Analyses were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. (D) Quantification of track duration time in Cav1-mCh cells, Dyn2-GFP-Cav1-mCh cells or GFP-EHD2-Cav1-mCh cells non-treated (grey bar) or treated with fusogenic liposomes containing cholesterol (black bar) for 20 minutes prior to imaging. Analysis were performed using Imaris software and track mean from at least 10 cells per condition are shown ± SEM. Significance was assessed using t test, ** p ≤0.01, *** p ≤0.001.

Article Snippet: Grids were immunolabelled using primary antibodies to caveolin-1 (BD Biosciences), rabbit anti-EHD2 ( ) or to dynamin (mouse HUDY1, Sigma), followed by secondary labelling with goat anti-mouse gold and goat anti-rabbit gold.

Techniques: Imaging, Software